In DNA replication, what strand is mRNA produced from?

mRNA is synthesized from the anti-sense strand of DNA during the process of transcription. The anti-sense strand, also referred to as the template strand, serves as the basis for mRNA synthesis, where complementary base pairing occurs—adenine (A) pairs with uracil (U) in RNA, and cytosine (C) pairs with guanine (G).

Understanding the roles of the different strands is crucial. The sense strand, or coding strand, has a sequence identical to the mRNA produced (except for the substitution of uracil for thymine). However, it is the anti-sense strand that RNA polymerase reads to produce the mRNA. This requires clarity on the processes of DNA replication and transcription, as well as the roles of leading and lagging strands, which refer specifically to the direction of DNA synthesis during replication rather than transcription.