Chargaff's Rule primarily highlights the relationship between which two nucleobases?

Chargaff's Rule is fundamental in understanding the base pairing in DNA and specifically states that in a given DNA molecule, the amount of adenine will equal the amount of thymine, and the amount of guanine will equal the amount of cytosine. This complementary nature of the nucleobases is crucial for the structure of DNA and its ability to replicate accurately.

In this context, adenine pairing with thymine exemplifies how Chargaff's Rule operates, as they form complementary pairs through hydrogen bonding. This pairing is not just a feature of base composition but is also essential for the integrity of genetic information being accurately copied during DNA replication and transcription processes. The pairing ensures that the genetic code is preserved and passed on to subsequent generations of cells.

Understanding that adenine pairs specifically with thymine highlights the importance of this relationship in the broader context of molecular biology and genetics, illustrating why the correct answer centers on these two nucleobases as representative of Chargaff's Rule.